Identification of new minisatellites loci in Arabidopsis thaliana |
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Authors: | Tourmente S; Lazreg A; Lafleuriel J; Tutois S; Cuvillier C; Espagnol M; Picard G |
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Institution: | UMR 6547 Biomove, Universite Blaise Pascal, 24 Avenue des Landais, F-63177 Aubiere Cedex, France; Corresponding author e-mail: tourmente@cicsum.univ-bpclemontfr |
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Abstract: | In order to characterize new CG-rich minisatellites present in the
Arabidopsis thaliana genome, a genomic library was
screened at low stringency with a probe containing nine repeated-units of a
minisatellite (CMs1) previously identified. Both minisatellites and
minisatellite-like elements were identified. The minisatellites, with a
tandemly-repeated structure, all contain the Arabidopsis
thaliana-core sequence previously defined (Tourmente
et al., 1994). Both minisatellite and
minisatellite-like sequences occur in the Arabidopsis
genome in low copy and are weakly polymorphic between ecotypes. The genetic
mapping of these markers has shown that they are dispersed on the genome.
YACs clones of the CIC library carrying these minisatellites and
minisatellite-like sequences were identified.Key words:
Arabidopsis thaliana, minisatellites, polymorphism
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