Lobster (Homarus americanus) striated muscle myosin. |
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| Authors: | J M Regenstein |
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| Affiliation: | Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, MA 02154. |
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| Abstract: | 1. Myosin from the thin-filament regulated flexor muscle of lobster contains 2 moles of each of 2 light chains. 2. The Lb 1 light chain of 19,000 daltons which can be removed by DTNB is heavier than the DTNB light chain of chicken. The Lb 2 light chain of 17,000 daltons can be removed with urea. 3. On electrophoresis in 8 M urea (pH 8.7) the Lb 2 light chain migrates with a mobility similar to that of chicken A2, but the Lb 1 migrates significantly faster than any of the chicken light chains. 4. In lobster, the DTNB treatment destroys the Ca and K-EDTA ATPase activity of lobster myosin. |
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