Importance of TP53 and RB in the repair of potentially lethal damage and induction of color junctions after exposure to ionizing radiation |
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Authors: | Franken N A P van Bree C ten Cate R van Oven C H Haveman J |
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Affiliation: | Department of Radiotherapy, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. N.A.Franken@amc.nl |
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Abstract: | Repair of potentially lethal damage (PLD) was investigated in cells with functional G1-phase arrest with wild-type TP53 and wild-type RB and in cells in which G1-phase arrest was abrogated by inactivation of TP53 or RB. Confluent cultures of cells were plated for clonogenic survival assay either immediately or 24 h after irradiation. Induction of color junctions, an exchange between a painted and unpainted chromosome, was studied in chromosomes 18 and 19 after irradiation with 4 Gy gamma rays. Significant repair of PLD was found in cells carrying both wild-type TP53 and wild-type RB. In cells in which TP53 or RB was inactivated, the survival curves from immediately plated and delayed-plated cells were not significantly different. The numbers of radiation-induced color junctions in chromosomes 18 and 19 were similar in all cell lines. From this study we conclude that a functional G1-phase arrest is important for repair of PLD and that TP53 and RB do not affect the frequencies of induction of color junctions in chromosome 18 or 19. |
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