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Evidence that two genomic species of Rhizobium are associated with Medicago truncatula |
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| Authors: | Sophie Rome Brigitte Brunel Philippe Normand Maria Fernandez Jean-Claude Cleyet-Marel |
| Institution: | (1) Laboratoire d'Ecologie Microbienne du Sol, URA-CNRS 1977, Université Lyon I, Bat 741, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, Cedex, France Fax: +33-72-431223 e-mail: sr@geometrie.univ-lyon1.fr, FR;(2) Laboratoire de Recherche sur les Symbiotes des Racines, ENSAM-INRA, 2 Place P. Viala, F-34060 Montpellier, Cedex 01, France , FR;(3) Unité de Formation et de Recherche de Science du Sol, ENSAM-INRA, 2 Place Viala, F-34060 Montpellier, Cedex 01, France , FR |
| Abstract: | Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization. Received: 1 August 1995 / Accepted: 13 October 1995 |
| Keywords: | Rhizobium meliloti Medicago truncatula PCR/RFLP 16S rDNA nifD-K region nodD1 gene nodD2 gene DNA/DNA hybridization |
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