Glycogen metabolism: a 13C-NMR study on the isolated perfused rat heart |
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Authors: | N Lavanchy J Martin A Rossi |
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Affiliation: | Laboratoire d''Endocrinologie Expérimentale et Clinique, Université de Liège, CHU B23-B-4000 Liège, Belgium |
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Abstract: | Glycogen synthesis from D-[1-13C]glucose was observed in the perfused rat heart by 13C-NMR spectroscopy at 62.9 MHz. The glycogenogenesis was stimulated by pretreatment of the animals with isoprenaline. Whereas in hearts from control rats the incorporation of D-[1-13C]glucose into the glycogen remained below the detection threshold, 5 min proton-decoupled 13C-NMR spectra revealed, in hearts from treated rats, a significant labelling of the glycogen within the first minutes of the perfusion and a further linear increase of the glycogen resonance for up to 25 min. This model was used to monitor the appearance of 13C-labelled lactate during ischemia. |
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Keywords: | Collagen Fibroblast Thyroid hormone Triiodothyronine |
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