Unique Properties of Nucleic Acid from <Emphasis Type="Italic">Bacillus subtilis</Emphasis> Phage SP-15 |
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Authors: | J MARMURM C BRANDON S NEUBORT M EHRLICH M MANDEL J KONVICKA |
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Institution: | 1.Department of Biochemistry,Albert Einstein College of Medicine,Bronx;2.Department of Biology,University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston,Houston |
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Abstract: | BACTERIOPHAGE SP-15 is a large, generalized transducing phage of Bacillus subtilis and B. licheniformis. The DNA extracted from the purified phage has unusual physical properties: its melting temperature (Tm) in 0.15 M NaCl, 0.015 M sodium citrate (SSC) is very low, 61.5° C and its buoyant density in neutral CsCl is very high, 1.761 g/ml.1. We describe here additional unique features of SP-15 DNA: the presence of (1) a new modified pyrimidine which partially replaces the thymine; (2) a compound which reacts with orcinol as a pentose; (3) alkali-sensitive phosphodiester bonds; and (4) glucose. |
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