Generation of two modified mouse alleles of the Hic1 tumor suppressor gene |
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Authors: | Pospichalova Vendula Tureckova Jolana Fafilek Bohumil Vojtechova Martina Krausova Michaela Lukas Jan Sloncova Eva Takacova Sylvia Divoky Vladimir Leprince Dominique Plachy Jiri Korinek Vladimir |
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Affiliation: | Department of Cell and Developmental Biology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic. |
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Abstract: | HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine "reporter" mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. |
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Keywords: | hypermethylated in cancer 1 Hic1 tumor suppressor gene targeting a conditional null allele citrine reporter mice placenta |
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