Cytogenetic analyses of a murine carcinoma cell line and six metastatic derivatives with different degrees of radioresistability |
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Authors: | Roudabeh J Jamasbi Ming-Qi Ye Tonya M Norvell |
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Institution: | (1) Departments of Medical Technology and Biological Sciences, Bowling Green State University, 504 Life Sciences Building, 43403 Bowling Green, Ohio |
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Abstract: | Summary We reported that a murine carcinoma (DEN3) and its six pulmonary metastases (M2, M4C, M4D, M4E, M4F, and M6) exhibited different
degrees of radioresistability (In Vitro Cell. Dev. Biol. 26:222–228; 1990). While the M2, M4C, M4E, and M4F cultured cells
survived up to 2.5 Gy, the cells of DEN3 and M6 tolerated up to 5.0 Gy, and the M4D cells could withstand up to 10.0 Gy of
X-irradiation. In the present investigation, the cytogenetic features of these cell lines were examined: (a) to determine
the degree of cytogenetic heterogeneity among these cell lines, and (b) to investigate whether any association between the
cytogenetic anomaly and the degree of radioresistability could be established. Heterogeneous cytogenetic aberrations were
detected in all of the above lines. Karyotype analysis of the M4D and M6 cell lines displayed both numerical and structural
abnormalities. The gain and loss of chromosomal copies were observed. Structural aberrations, such as translocation and deletion
appeared in both cell lines. However, correlation between the cytogenetic abnormality and the degree of radioresistability
was not demonstrated except for a dramatic reduction in one or more copies of the X-chromosome that occurred in 86% and 93%
of the M6 and M4D cells, respectively. The results suggest heterogeneous cytogenetic aberrations among these cell lines and
a possible association between the loss of X-chromosome and radioresistability of these tumor cells. |
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Keywords: | Diethylnitrosamine x rays cytogenetic X-chromosome |
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