Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale |
| |
Authors: | Milek Igor Cigic Blaz Skrt Mihaela Kaletunç Gönül Ulrih Natasa Poklar |
| |
Institution: | Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Slovenia. |
| |
Abstract: | Growth of Aeropyrum pernix, the first reported aerobic neutrophilic hyperthermophilic archaeon, was investigated under different cultivation parameters. Different sources of seawater, pH, and the cultivation methods were tested with the aim to improve the biomass production. A 1-L glass flask fitted with a condenser and air diffuser was used as a bioreactor. The optimum conditions for maximizing A. pernix biomass were obtained when Na2S2O3.5H2O (1 g/L) with added marine broth 2216 at pH 7.0 (20 mmol HEPES buffer/L) was used as a growing medium in a 1-L flask. The biomass production was 0.45 g dry cell mass/L in 40 h under the optimum conditions, which is more than the 0.42 g dry cell mass/L in 60 h previously obtained. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|