| 粘质沙雷氏菌几丁质酶chiB基因的克隆与序列分析 |
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| 引用本文： | 叶辉,程备久,朱苏文,甘德芳,冯春.粘质沙雷氏菌几丁质酶chiB基因的克隆与序列分析[J].激光生物学报,2007,16(3):299-303. |
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| 作者姓名： | 叶辉 程备久 朱苏文 甘德芳 冯春 |
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| 作者单位： | 安徽农业大学,生物技术中心,安徽,合肥,230036;安徽农业大学,生命科学学院,安徽,合肥,230036;安徽农业大学,园艺学院,安徽,合肥,230036;安徽农业大学,农业因子实验总场管理中心,安徽,合肥,230036 |
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| 基金项目： | Scientifical Item of Education Department of Anhui Province(No.2006KJ213B) |
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| 摘 要： | 采用改进的方法提取粘质沙雷氏菌基因组DNA,通过PCR扩增,得到大小为1 500 bp特异性DNA片段(chiB基因),以pUC18质粒构建了pUC-ch iB克隆载体,转化至感受态细胞E.coliDH5α培养,并筛选出重组质粒。经测序分析,证明克隆片段与文献报道相一致。
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| 关 键 词： | 几丁质酶 chiB基因 克隆 序列分析 |
| 文章编号： | 1007-7146（2007）03-0299-05 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene |
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| YE Hui,CHENG Bei-jiu,ZHU Su-wen,GAN De-fang,FENG Chun.Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene[J].ACTA Laser Biology Sinica,2007,16(3):299-303. |
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| Authors: | YE Hui CHENG Bei-jiu ZHU Su-wen GAN De-fang FENG Chun |
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| Institution: | Anhui Agricultural University a. Biotechnology Center; b. College of Life Science ; c. College of Horticulture; d. Administratire Center of Agricuhural Gardens, Hefei 230036, Anhui, China |
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| Abstract: | The genome DNA was extracted from serratia marcescens by improved method .A special fragment about 1 500 bp length was cloned from serratia marcescens genome DNA by Polymerasw Chain Reaction (PCR) amplification. Vector Puc-CHIb was constructed through ligating the fragment into the plasmid pUC18 and transformed into E. Coli DH5α. Through screening of recombinants and sequence analysis of it, the result showed that the cloned DNA fragment was chitinase chiB gene of Serratia marcescens which was the same as reported. |
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| Keywords: | chitinase chiB gene cloning sequence analysis |
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