正常及损伤后修复的内皮细胞合成的蛋白聚糖

用自制尼龙刷将培养至汇合的人脐静脉内皮细胞刮伤后,造成规则的内皮细胞缺失区。继续培养可见,原有的内皮钿胞很快迁移到缺失区,并分裂增殖。约48小时新生的内皮细胞即将缺失区全部修复而形成新的汇合单层。以DEAE-Sephacel离子交换及Sepharose 6B凝胶过滤柱层析分析损伤后修复的内皮细胞合成的蛋白聚糖时发现所合成的蛋白聚糖总量减少;硫酸乙酰肝素蛋白聚糖合成相对减少、而硫酸软骨素及/或硫酸皮肤素蛋白聚糖合成相对增多。说明:伴随着内皮细胞的损伤后修复其蛋白聚糖的合成也有质和量的改变。

Proteoglycans Synthesized by Normal and Regrown Endothelial Cells

The monolayer of cultured confluent endothelial cell (EC) from human umbilical vein was disrupted by multi-scratch with a nylon brush. This treatment resulted in regular denuded areas on the monlayer. EC migrated to the denuded areas and proliferated following subsequent culturing of the multi-scratched monolayer. After 48 hours, the denuded areas were filled by newly formed neoendothelial cells. Analyses of the proteoglycans (PGs) synthesized and secreted by the newly formed endothelial cell monolayer and normal confluent EC monolayer on DEAE-Sephacel ion exchange and Sepharose 6B gel filtration chromatography revealed that the total amount of PGs decreased among which the relative percentage of heparan sulfate PG (HSPG) decreased while that of chondroitin sulfate/dermatan sulfate PG (CS/DSPG) increased. These results suggest that there are quantitative and qualitative changes in PG synthesis accompanying the regeneration of the endothelial monolayer.