Capillary electrophoresis assay of alanine:glyoxylate aminotransferase activity in rat liver |
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Authors: | Nishijima Saori Sugaya Kimio Morozumi Makoto Hatano Tadashi Ogawa Yoshihide |
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Affiliation: | Department of Urology, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Okinawa Nishihara 903-0215, Japan. k998739@med.u-ryukyu.ac.jp |
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Abstract: | We measured hepatic alanine:glyoxylate aminotransferase (AGT) activity using capillary electrophoresis. After rat liver homogenate was incubated in the presence of substrates and pyridoxal 5'-phosphate, the pyruvate and glycine produced by AGT were measured. The AGT activity was 10.02+/-0.31 micro mol pyruvate/h/mg protein and 10.21+/-0.15 micro mol glycine/h/mg protein. This method is relatively simple and shows superior sensitivity, allowing the measurement of enzyme activity in 5 micro g of protein. Therefore, it appears to be suitable for laboratory use and may also have advantages for measuring AGT activity in liver biopsy specimens. |
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Keywords: | Alanine:glyoxylate aminotransferase Enzymes |
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