Functionalized N-aryl azetidinones as novel mechanism-based inhibitors of neutrophil elastase |
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| Authors: | M Wakselman R Joyeau R Kobaiter N Boggetto I Vergely J Maillard V Okochi J J Montagne M Reboud-Ravaux |
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| Institution: | Centre National de la Recherche Scientifique, Centre d'Etudes et de Recherches de Chimie Organique Appliquée, Laboratoire de Chimie Organique Biologique, Thiais, France. |
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| Abstract: | A functionalized N-aryl azetidinone has been shown to inactivate human leukocyte elastase (HLE) and porcine pancreatic elastase (PPE) by an enzyme-mediated process. The inactivation is characterized by the following kinetic constants at pH 8.0 and 37 degrees C: kinact = 0.035 s-1, KI = 1.2 x 10(-4) M for HLE, 0.08 s-1 and 2.7 x 10(-4) M for PPE, respectively. Two parent molecules devoid of the latent leaving group failed to inactivate HLE and PPE and behaved as substrates of these enzymes. A suicide mechanism is postulated involving the formation of an acyl-enzyme and the simultaneous unmasking of a latent quinonimmonium methide ion which irreversibly reacts with an active site nucleophile. Moreover, the inhibitor is still effective at inhibiting elastase preabsorbed onto elastin. |
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