Purification of lipopolysaccharide from strains of Yersinia enterocolitica belonging to serogroups 03 and 09 |
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Authors: | Henrik Chart Bernard Rowe |
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Affiliation: | Division of Enteric Pathogens, Central Public Health Laboratory, London, U.K. |
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Abstract: | Lipopolysaccharide (LPS) was purified from strains of Yersinia enterocolitica belonging to serogroups 03 and 09, by three methods, and analysed by SDS-PAGE and silver staining for carbohydrate. SDS-PAGE of LPS prepared from whole-cells by digestion with proteinase-K, produced profiles containing high molecular mass LPS and a lower molecular mass region migrating as discrete bands. LPS prepared from strains belonging to serogroup 03, using a hot-phenol procedure alone was found to contain cellular proteins, and LPS prepared from strains of serogroup 09, by this method, did not contain high molecular mass carbohydrate. A novel method of preparing LPS by digesting bacterial outer membranes with proteinase-K prior to hot-phenol extraction produced protein-free LPS from strain of Y. enterocolitica 03 and high molecular mass LPS from strains belonging to serogroup 09. |
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Keywords: | Yersinia enterocolitica Lipopolysaccharide O-antigen Proteinase-K SDS-PAGE Silver staining |
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