Cell population dynamics (apoptosis, mitosis, and cell-cell communication) during disruption of homeostasis |
| |
Authors: | Wilson M R Close T W Trosko J E |
| |
Affiliation: | Department of Pediatrics and Human Development, College of Human Medicine, East Lansing, Michigan 48824, USA. wilson31@pilot.msu.edu |
| |
Abstract: | The sequence of events involved in maintenance of homeostasis must encompass mechanisms within single cells as well as interactions between cells within a population. To investigate the interaction among these inter- and intracellular mechanisms, disruption of homeostasis by serum deprivation was performed in WB-F344, a normal diploid epithelial cell line. Changes in cell-cell communication (gap junction function) at the population level and in individual cells were monitored using the scrape load/dye transfer and fluorescence redistribution after photobleaching assays. Apoptosis and mitosis were measured using internucleosomal DNA ladder assays and fluorescence-activated cell sorting. The results indicate that a common element in early apoptosis and early mitosis is sustained gap junction function. As cell life (mitosis) and cell death (apoptosis) progressed, a common process of change in gap junction function occurred. A transient stimulation of mitosis concomitant with increased apoptosis was also observed during serum deprivation. Gap junctions may play a regulatory role during initiation of these opposite yet equally important mechanisms of maintaining homeostasis. This model system is useful for further studies on the relationships among inter- and intracellular mechanisms of homeostasis. |
| |
Keywords: | programmed cell death cell–cell communication connexin 43 bystander effect stem cells cancer epithelial |
本文献已被 ScienceDirect PubMed 等数据库收录! |