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几种蛇毒与鲎试剂产生凝胶反应的实验研究
引用本文:蒋三元,李景新,张健民.几种蛇毒与鲎试剂产生凝胶反应的实验研究[J].蛇志,2009,21(4):261-262.
作者姓名:蒋三元  李景新  张健民
作者单位:广东省江门市新会区中医院,广东江门,529100
摘    要:目的探讨蛇毒能否与鲎试剂产生凝胶反应.建立中药及有效成分抗蛇毒作用的试验途径。方法采用鲎试剂试管凝胶反应法。结果不同的蛇毒能使鲎试剂产生凝胶反应的浓度不同,眼镜蛇毒与竹叶青蛇毒为5μg/ml.五步蛇毒0.32μg/ml.蝮蛇毒37.5μg/ml,蝰蛇毒2.5pg/ml。结论蛇毒能与鲎试剂产生凝胶反应。

关 键 词:蛇毒  鲎试剂  凝胶反应

Experimental Study of Several Snakes Venom and TAL-gel-clot reaction
JIANG San-yuan,LI Jing-xin,ZHANG Jian-min.Experimental Study of Several Snakes Venom and TAL-gel-clot reaction[J].Journal of Snake,2009,21(4):261-262.
Authors:JIANG San-yuan  LI Jing-xin  ZHANG Jian-min
Institution:(Xinhui Hospital of Traditional Chinese Medicine, Jiangmen, Guangdong, 529100, China)
Abstract:Objective To investigate whether venom reacts with TAL to produce gel-clot, and establish effective anti-venin assays of the active ingredients of Chinese medicine. Methods TAL-gel reaction in vitro. Results Different types of snake venom react to TAL with different concentrations of cobra and trimeresurus for 5μg/ml, acutus 0. 32 μg/ml, agkistrodon 37. 5μg/ml, viper 2.5 μg/ml. Conclusion Snake venom can reacts with TAL to produce gelclot.
Keywords:snake venom  taehypleus amebocyte lysate (TAL)/limulus amebocyte lysate (LAL)/limulus reagent  get-clot reaction
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