Application of Surface-Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells |
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Authors: | Hope T. Beier Christopher B. Cowan I-Hsien Chou James Pallikal James E. Henry Melodie E. Benford Joseph B. Jackson Theresa A. Good Gerard L. Coté |
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Affiliation: | (1) Department of Biomedical Engineering, Texas A&M University, 337 Zachry Engineering Center, 3120 TAMU, College Station, TX 77843-3120, USA;(2) Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD, USA;(3) Nanospectra Biosciences, Inc., Houston, TX, USA;(4) Present address: Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA, USA |
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Abstract: | Currently, no methods exist for the definitive diagnosis of AD premortem. β-amyloid, the primary component of the senile plaques found in patients with this disease, is believed to play a role in its neurotoxicity. We are developing a nanoshell substrate, functionalized with sialic acid residues to mimic neuron cell surfaces, for the surface-enhanced Raman detection of β-amyloid. It is our hope that this sensing mechanism will be able to detect the toxic form of β-amyloid, with structural and concentration information, to aid in the diagnosis of AD and provide insight into the relationship between β-amyloid and disease progression. We have been successfully able to functionalize the nanoshells with the sialic acid residues to allow for the specific binding of β-amyloid to the substrate. We have also shown that a surface-enhanced Raman spectroscopy response using nanoshells is stable and concentration-dependent with detection into the picomolar range. |
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Keywords: | Surface-enhanced Raman spectroscopy SERS Raman spectroscopy Nanoshells β -amyloid Alzheimer’ s disease Congo red Self-assembled monolayer |
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