Cytoplasmic dynein and microtubule transport in the axon: The action connection |
| |
Authors: | K Kevin Pfister |
| |
Institution: | (1) Cell Biology Department, School of Medicine, University of Virginia, PO Box 800732, 22908-0732 Charlottesville, VA |
| |
Abstract: | The neuron uses two families of microtubule-based motors for fast axonal transport, kinesin, and cytoplasmic dynein. Cytoplasmic
dynein moves membranous organelles from the distal regions of the axon to the cell body. Because dynein is synthesized in
the cell body, it must first be delivered to the axon tip. It has recently been shown that cytoplasmic dynein is moved from
the cell body along the axon by two different mechanisms. A small amount is associated with fast anterograde transport, the
membranous organelles moved by kinesin. Most of the dynein is transported in slow component b, the actin-based transport compartment.
Dynactin, a protein complex that binds dynein, is also transported in slow component b. The dynein in slow component b binds
to microtubules in an ATP-dependent manner in vitro, suggesting that this dynein is enzymatically active. The finding that
functionally active dynein, and dynactin, are associated with the actin-based transport compartment suggests a mechanism whereby
dynein anchored to the actin cytoskeleton via dynactin provides the motive force for microtubule movement in the axon. |
| |
Keywords: | Dynein dynactin actin microtubule axonal transport motor protein |
本文献已被 PubMed SpringerLink 等数据库收录! |
|