Glutamine decomposition in DMEM: Effect of pH and serum concentration

Summary The pH and serum dependence of the glutamine decomposition rate constant, K_gln, in Dulbecco's Modified Eagle's Medium, DMEM, was determined. The findings indicate that K_gln increases with increase in pH and fetal calf serum (FCS) concentration in DMEM. At a constant pH of 7.25, K_gln increases from 5.7×10^–4 to 13.2×10^–4 h^–1 as FCS content of the medium increases from 0.0 to 10.0 (% v/v). Moreover, at a constant FCS of 10 (% v/v), K_gln increases from 11.5×10^–4 to 33.6×10^–4 h^–1 as pH of the medium increases from 7.2 to 7.6.