Paralytic peptide activates insect humoral immune response via epidermal growth factor receptor |
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| Affiliation: | 1. Agriculture and Agri-Food Canada, Saskatoon, SK, Canada;2. Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada;3. Department of Plant Protection, College of Agriculture, University of Ankara, Ankara, Turkey;4. Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada;1. Laboratorio de Biología Celular y Molecular, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México;2. Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California (UABC), Av. Tecnológico s/n, Mesa de Otay, Tijuana, Baja California C.P. 22390, México;3. Laboratorio de Neurofarmacología Marina, Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM), Blvd. Juriquilla 3001, Juriquilla, Querétaro C.P. 76230, México;4. Laboratorio de Ecofisiología de Organismos Acuáticos, Departamento de Biotecnología Marina, Centro de Investigación Científica y Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana #3918, Ensenada, Baja California C.P. 22860, México;1. Departamento de Bioquímica, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;2. Centro de Biotecnologia e Química Fina – Laboratório Associado de Estado, Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal;3. Biotério, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;4. Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal;5. Departamento de Farmacologia e Terapêutica, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;6. MedinUP, Center for Drug Discovery and Innovative Medicines, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;7. CINTESIS, Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;8. Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Júlio Amaral de Carvalho n° 245, 4200-135 Porto, Portugal;1. Dept. Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitätsstr. 150, 447801 Bochum, Germany;2. Department of Animal Ecology I and BayCEER, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany;3. Department of Biology II, Ludwig-Maximilians-University Munich, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany;1. Mass Spectrometry Centre, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;2. QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;3. Institute for Biomedicine—iBiMED, Health Sciences Program, University of Aveiro, Portugal |
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| Abstract: | Paralytic peptide (PP) activates innate immunity of silkworm Bombyx mori, inducing production of anti-microbial peptides (AMPs) and phagocytosis-related proteins; however the signal pathways of PP-dependent immune responses are not clear. In present study, we characterized BmE cells as a PP-responsive cell line by examining the expression of AMP genes and activation of p38 mitogen-activated protein kinase (p38 MAPK) under PP stimulation, and we also found PP directly binds to BmE cell membrane. Then we found that PP-dependent expression of AMP genes is suppressed by tyrosine kinase inhibitor (genistein) both in BmE cells and in fat body of silkworm larvae. Moreover, the specific tyrosine kinase epidermal growth factor receptor (EGFR) inhibitor (AG1478) attenuates PP-induced expression of AMP genes in BmE cells and fat body of silkworm and RNA interference (RNAi) to BmEGFR also suppresses PP-induced expression of AMP genes. Furthermore, the PP-induced p38 MAPK phosphorylation is inhibited by AG1478. Our results suggest that BmE cells can be used as a cell model to investigate the signal pathway of PP-dependent humoral immune response and receptor tyrosine kinase EGFR/p38 MAPK pathway is involved in the production of AMPs induced by PP. |
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| Keywords: | Paralytic peptide Antimicrobial peptides Epidermal growth factor receptor p38 mitogen-activated protein kinase Humoral immune response |
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