Effects of BMP-2 compound with fibrin on osteoporotic vertebral fracture healing in rats |
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Authors: | Xiao Ouyang Yunzhi Ding Li Yu Feng Xin Xiaowei Yang Peng Sha Songming Tong Qi Cheng Yiqi Xu |
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Institution: | Department of Orthopedic Surgery, Xuzhou Third Hospital, Affiliated Xuzhou Hospital of Jiangsu University, Xuzhou, China |
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Abstract: | Objectives:To investigate the effects of bone morphogenetic protein-2 (BMP-2) compound with fibrin on osteoporotic vertebral fracture healing in rats.Methods:For the present study 160 Specific-Pathogen Free 32-week-old female Sprague-Dawley rats were used. 120 rats were randomly divided in three groups (experimental, model and sham operation group- n=40 per group) and were ovariectomized to establish the osteoporosis model. 40 rats served as a control group without treatment. The expression of BMP-2 in the fracture zone at the 4th, 6th, 8th, and 12th weeks was detected by qRT-PCR. The expression of BALP and CTX-I in serum at the 12th week was detected by Elisa.Results:At week 8, the morphology of the sham operation group was the same and the fracture healing occurred more slowly than in the other groups. At week 12, the expression of BMP-2 in the model group was significantly higher than that in the other three groups (p<0.05). At week 12, the maximum load, maximum strain, and elastic modulus of model group were significantly lower than those of the other three groups.Conclusions:BMP-2 compound with fibrin can enhance the timing and quality of bone fracture healing in rats. |
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Keywords: | Bone Morphogenetic Protein-2 Fibrin Fracture Healing Osteoporosis Vertebral Fracture |
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