Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure |
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Authors: | Choi Hee-Jung Park-Snyder Shaun Pascoe Lauren T Green Kathleen J Weis William I |
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Institution: | Department of Structural Biology, Stanford University School of Medicine, 299 Campus Drive West, Stanford, California 94305, USA. |
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Abstract: | Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site. |
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