In vivo intracellular cytokine production by leukocytes during haemodialysis |
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Authors: | Rousseau Y Haeffner-Cavaillon N Poignet J L Meyrier A Carreno M P |
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Institution: | 1. Farmacología y Toxicología, Centro de Diagnóstico Biomédico, IDIBAPS, Hospital Clínico, Universidad de Barcelona, c/Villarroel 170, 08036 Barcelona, Spain;2. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), c/Sinesio Delgado 4, 28029 Madrid, Spain;3. Unidad de Hígado, Hospital Clínico, IDIBAPS, c/Villarroel 170, 08036 Barcelona, Spain;4. Servicio de Inmunología, Hospital Universitario Marqués de Valdecilla-IFIMAV, c/Avda. Valdecilla 25, 39008 Santander, Spain;5. Servicio de Inmunología, Hospital Universitario Virgen Arrixaca, Ctra. Madrid-Cartagena s/n, 30120 El Palmar, Murcia, Spain;6. Departamento de Inmunología, Hospital Universitario “12 de Octubre”, Universidad Complutense, c/Avda de Córdoba s/n, 28041 Madrid, Spain;7. Unidad Docente de Medicina Familiar y Comunitaria de Santander, Servicio Cántabro de Salud, c/Las Cruces, 39005 Santander, Spain;8. Servicio de Medicina Digestiva Hospital Universitario Virgen Arrixaca, Ctra. Madrid-Cartagena s/n, 30120 El Palmar, Murcia, Spain;9. Servicio de Nefrología, Hospital Universitario “12 de Octubre”, Universidad Complutense, c/Avda de Córdoba s/n, 28041 Madrid, Spain;10. Unidad de Trasplante Renal, Servicio de Nefrología, Fundació Puigvert, c/Cartagena 340-350, 08025 Barcelona, Spain;11. Servicio de Nefrología, Hospital del Mar, c/Passeig Marítim de la Barceloneta, 25-29, 08003 Barcelona, Spain |
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Abstract: | Several chronic inflammatory changes undergone during chronic haemodialysis are associated with increased pro-inflammatory cytokine production. Although generation of anaphylatoxins has been incriminated in the untoward effects of haemodialysis, it is still debated whether anaphylatoxins stimulate monocyte secretion of TNF-alpha and IL-1. We demonstrate that peripheral mononuclear cells isolated from healthy controls and cultured with complement-activated autologous serum or recombinant C5a induced high levels of IL-1, IL-1ra, IL-8 and MCP-1, low levels of TNFalpha and sTNFRII but no IL-10 and MIP-1alpha. Cytokine production by leukocytes was investigated by FACS analysis in six patients dialysed consecutively with three equivalent low permeability membranes known to activate the complement to different degrees: polysulfone (F6HPS), cellulose acetate (CA) and cuprophane (CP). Percentage of leukocytes expressing IL-1, IL-1ra, TNF-alpha and IL-8 is increased in patients dialysed with CP. Moreover, we show for the first time that haemodialysis is associated with the production of cytokines by circulating neutrophils. Predialysis plasma levels of MCP-1 and TNFRII did not increase during the dialysis session at the time when anaphylatoxin generation was highest. Dialysis with membranes that activate the complement to a high extent induce activation of leukocytes which may explain chronic complications associated with dialysing with CP. |
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