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Genetic control of chalcone-flavanone isomerase activity in Callistephus chinensis
Authors:B. Kuhn  G. Forkmann  W. Seyffert
Affiliation:(1) Institut für Biologie II, Lehrstuhl für Genetik der Universität Tübingen, Auf der Morgenstelle 28, D-7400 Tübingen, Federal Republic of Germany
Abstract:A mutant blocked in anthocyanin synthesis leads to an accumulation of 4,2prime,4prime,6prime-tetrahydroxy-chalcone-2prime-glucoside (isosalipurposide) in blossoms of Callistephus chinesis (L.) Nees, whereas in geno-types with the wild-type allele, higher oxidized flavonoids and anthocyanins are synthesized. Measurements of chalcone-flavanone isomerase activity of 18 lines of Callistephus chinensis showed a clear correlation between accumulation of chalcone in the recessive genotypes (ch ch) and deficiency of this enzyme activity. Both the chemogenetic and the enzymologic evidence lead to the following conclusions: 1. The first product of the synthesis of the flavonoid skeleton should be tetrahydroxychalcone.-2. The chalcone-flavanone isomerase catalyzes the formation of flavanone from chalcone in a stereospecific way and there-with furnishes the substrate for the further reactions in the flavonoid biosynthesis.Abbreviations EGME ethylene glycol monomethyl ether - HOAc acetic acid - MeOH methanol - PVP polyvinylpyrrolidone - TBA tert. butanol-acetic acid-water, 3:1:1 - TLC thin-layer chromatography
Keywords:Callistephus  Chalcone-flavanone isomerase  Genetic block  Flavonoid biosynthesis  4,2  /content/uu3830p68256438v/xxlarge8242.gif"   alt="  prime"   align="  BASELINE"   BORDER="  0"  >,4  /content/uu3830p68256438v/xxlarge8242.gif"   alt="  prime"   align="  BASELINE"   BORDER="  0"  >,6  /content/uu3830p68256438v/xxlarge8242.gif"   alt="  prime"   align="  BASELINE"   BORDER="  0"  >-Tetrahydroxychalcone-2  /content/uu3830p68256438v/xxlarge8242.gif"   alt="  prime"   align="  BASELINE"   BORDER="  0"  >-glucoside
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