5种中国苏云金芽孢杆菌的伴孢晶体蛋白基因分析

利用聚合酶联反应(PCR)和聚丙烯酰胺凝胶电泳(SDS-PAGE)技术分析了5种中国苏云金杆菌制剂菌株的伴孢晶体蛋白及其基因组成。结果发现，5种菌株均含有cry1Aa和/或c和/或d和/或b基因，只有Bt+Virus菌株含有cry1Ab基因，cry1A基因编码的伴孢晶体蛋白分子量约为130 kD；仅有JS-Bt C菌株含有cry1B基因，其编码的伴孢晶体蛋白分子量约为138 kD；除HB Bt C菌株外，其余4个菌株均含有cry2Aa和/或b基因，这类基因编码分子量为70 kD的伴孢晶体蛋白；所有5个菌株都含有cry1I基因，其编码的伴孢晶体蛋白分子量应为81.2 kD，但实验中未曾检测到cry1I基因的表达；所有的菌株都不含有cry1C和cry1D基因。

Analysis of five Chinese Bacillus thuringiensis formulations for the endotoxin components

In this paper, we investigated five Bacillus thuringiensis (Bt) strains from four major manufacturers of Bt products in China for the endotoxin components by PCR and SDS PAGE. All five strains harbour cry 1Aa and/or c , and/or d , and/or e genes, only the Bt Virus strain has cry1Ab gene. The cry1A class of genes encodes for insecticidal crystal proteins with MW of 130 kD and toxic to Lepidoptera. The JS Bt C strain contains the cry1B gene, which encodes for a 138 kD crystal protein, potentially toxic to both Lepidoptera and Coleoptera. Four of the five strains (HB Bt P, Bt Virus, JS Bt P, and JS Bt C) harbour the cry2Aa and/or b genes, which encode 70 kD crystal proteins toxic to Lepidoptera. All of the five strains have the cry1I gene which appears to be cryptic in all the strains. None of the five strains harbours the cry1C and cry1D genes.