The isolation of high molecular weight DNA from wheat,barley and rye for analysis by pulse-field gel electrophoresis |
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Authors: | Wing Y. Cheung Michael D. Gale |
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Affiliation: | (1) Cambridge Laboratory, Colney Lane, NR4 7UJ Norwich, UK |
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Abstract: | A method is presented for the preparation of large DNA molecules from protoplasts embedded in agarose blocks of three different cereals-hexaploid bread wheat (Triticum aestivum), barley (Hordeum vulgare) and rye (Secale cereale). Pulse-field gel electrophoresis (PFGE) analysis of these DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA molecules was greater than 6 Mb. DNA samples prepared by this method were shown to be useful for restriction analysis using both frequent and rare cutting enzymes. |
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Keywords: | cereals high molecular weight DNA pulse-field gel electrophoresis |
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