Inhibition effect of pcDNA-tum-5 on the growth of S180 tumor |
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Authors: | Yanjie You Xiaochang Xue Meng Li Xin Qin Cun Zhang Weihua Wang Changli Giang Shouzhen Wu Yan Liu Wenhua Zhu Yonggang Ran Zhen Zhang Wei Han Yingqi Zhang |
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Institution: | (1) Biotechnology Center of The Fourth Military Medical University, 17 Changle West Road, 710032 Xi’an, People’s Republic of China;(2) State Key Laboratory of Cancer Biology, 17 Changle West Road, 710032 Xi’an, People’s Republic of China |
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Abstract: | Tumor growth and metastasis depend on vessel formation, and inhibition of angiogenesis of tumor by production of anti-angiogenic
drugs should be a promising approach for cancer therapy. Tumstatin is an angiogenesis inhibitor. The anti-angiogenic activity
of tumstatin is localized to the 54–132 amino acids. The gene fragment encoding amino acids 45–132 of tumstatin (tum-5) was
subcloned into pcDNA3.1 (pcDNA-tum5). Tum-5 protein could be expressed and secreted in CHO cells after transfection. The conditioned
medium (containing tum-5 protein) from the transfectant has an anti-angiogenic effect on HUVEC cells in vitro. The anti-tumor
effect of pcDNA-tum5 on mice bearing S180 tumors was evaluated. The results showed that pcDNA-tum-5 has a significant inhibition
activity in the growth of the tumors. This study suggests that the gene delivery of tum-5 may be an effective strategy for
cancer therapy. |
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Keywords: | Tumstatin Gene therapy Anti-tumor effect Anti-angiogenic effect |
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