Comparison of genetic variability between Czech and foreign isolates of phytopathogenic bacteria Clavibacter michiganensis subsp. sepedonicus by Rep-PCR technique |
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| Authors: | Fousek J Mráz I Petrzik K |
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| Institution: | (1) Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czechia;(2) Faculty of Biological Sciences, University of South Bohemia, 370 05 České Budějovice, Czechia |
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| Abstract: | Repetitive-sequence-based polymerase chain reaction (Rep-PCR) method was used for analysis of genetic variability among bacterial
populations from different world locations. Collection of 26 Czech and 13 foreign strains ofClavibacter michiganensis subsp.sepedonicus was amplified using BOX primer targeting to repetitive motif occurring in eubacterial genomes. Genetic fingerprints were
visually compared and statistically evaluated by cluster analysis. Genetic similarity was estimated to be approximately 80%
among all tested strains. Populations of these bacteria seem to be highly homogeneous; potential influence of geographic origin
was not confirmed. |
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