Separation of immunomodulatory effects of mannan from Candida albicans into stimulatory and suppressive components |
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Authors: | J E Domer P W Stashak K Elkins B Prescott G Caldes P J Baker |
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Affiliation: | 2. CIBER de Enfermedades Respiratorias (CIBERES), Madrid, Spain;3. Attana, Stockholm, Sweden;4. Instituto de Agrobiotecnología, CSIC-UPNa-Gobierno Navarra, Mutilva, Spain |
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Abstract: | Mannan extracted from Candida albicans was studied for its immunomodulatory activity on in vivo antibody responses to type III pneumococcal polysaccharide (SSS-III), a helper-T-cell-independent antigen, and to sheep erythrocytes (SRBC), a helper-T-cell-dependent antigen. In some studies, the antibody response to SSS-III was converted to a helper-T-cell-dependent response by attaching it to a carrier (horse erythrocytes, HRBC); this complex then was used to immunize mice primed with a subimmunogenic dose of HRBC. Mannan enhanced the antibody response to both SSS-III and SRBC when administered at the same time or 1 or 2 days after immunogen. However, when both mannan and SSS-III were coated onto HRBC for immunization, either enhancement or suppression was noted; the effect depended upon the amount of mannan used. Larger amounts stimulated, whereas smaller amounts suppressed, the antibody response to SSS-III. The enhancing and suppressive components of mannan could be separated by molecular size or charge by chromatography on Sepharose 4B or on DEAE-Sephadex A-50 columns, indicating that mannan extracts contain individual components having opposing immunomodulatory properties. These components can be separated on the basis of molecular size and charge. |
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