Isolation and characterization of eleven microsatellite loci in small abalone, Haliotis diversicolor Reeve |
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| Authors: | Xin Zhan Hai-Yan Hu Cai-Huan Ke Song-Nian Hu De-Xiang Wang Fei Chen |
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| Institution: | (1) State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science, Xiamen University, 361005 Xiamen, China;(2) Key Laboratory of Genome Information and Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, 100029 Beijing, China;(3) Graduate University of Chinese Academy of Sciences, 100029 Beijing, China |
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| Abstract: | Eleven novel microsatellite markers were isolated from small abalone, Haliotis diversicolor Reeve. These loci were tested on 22 individuals from two different geographic populations. We identified a total of 162 alleles
from the 11 microsatellite loci. All of the loci were highly polymorphic. Polymorphism information content (PIC) is ranging
from 0.7276 to 0.9163. Observed and expected heterozygosities ranged from 0.2727 to 1.0000 and from 0.7738 to 0.9429, respectively.
Three loci deviated significantly from Hardy–Weinberg equilibrium. No pairs of loci displayed linkage disequilibrium. These
polymorphic markers will be used to analyze population structure, genetic diversity and construct a genetic linkage map.
Xin Zhan and Hai-Yan Hu contribute equally to this study. |
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| Keywords: | Magnetic beads enrichment Microsatellite markers Haliotis diversicolor Reeve Genetic linkage map |
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