The effect of vitamin D3 metabolites on membrane proteins of chick duodenal brush borders. |
| |
Authors: | S Moriuchi H F Deluca |
| |
Institution: | Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706 U.S.A. |
| |
Abstract: | Chick intestinal brush border proteins were examined by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate. Following injection of 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3, a large molecular weight protein present in the vitamin D-deficient brush borders diminishes and a larger protein appears. This change occurs before calcium binding protein can be detected by Chelex assay and prior to the increase in total alkaline phosphatase but correlates closely with increased intestinal calcium absorption in response to the metabolites. The two brush border proteins have been solubilized with n-butanol and partially characterized. The vitamin D-deficient protein has a molecular weight of about 200,000 and has alkaline phosphatase activity but no detectable calcium binding activity. The protein which appears in response to metabolites has a molecular weight of 230,000, binds calcium, and also has alkaline phosphatase activity. |
| |
Keywords: | To whom all correspondence should be addressed |
本文献已被 ScienceDirect 等数据库收录! |
|