Development and fine mapping of three co-dominant SCAR markers linked to the <Emphasis Type="Italic">M/m</Emphasis> gene in the cucumber plant (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.) |
| |
Authors: | Zheng Li Junsong Pan Yuan Guan Qianyi Tao Huanle He Longting Si Run Cai |
| |
Institution: | (1) Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai, China;(2) Department of Horticulture, Shenyang Agriculture University, Shenyang, China |
| |
Abstract: | Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to
environmental factors and plant hormones, three major genes—F/f, M/m, and A/a—regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified
polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated
with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully
developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers
S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals),
the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Z. Li and J. Pan contribute equally to this article. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|