In vitro propagation of herbaceous peony (paeonia lactiflora pall.) by a longitudinal shoot-split method |
| |
Authors: | Takashi Hosoki Michiko Ando Takehito Kubara Morihiko Hamada Masato Itami |
| |
Institution: | (1) Laboratory of Vegetable and Ornamental Horticulture, Faculty of Agriculture, Shimane University, Nishikawatsu-cho 1060, 690 Matsue, Japan |
| |
Abstract: | A procedure for the clonal propagation ofPaeonia lactiflora Pall. cvs. Takinoyosooi and Sarah Bernhardt through shoot tip culture is described. Half strength Murashige and Shoog (1962) medium supplemented with 0.5 mg/l 6-benzylaminopurine plus 1 mg/l gibberellic acid promoted formation and growth of axillary buds. Continuous shoot multiplication was achieved by vertically splitting the shoot axis and subsequent division of elongated axillary shoots every 36 days. High frequency (57–100%) of rooting was obtained on paper-bridge liquid medium supplemented with 1 mg/l indole-3-butyric acid. Half of the rooted plantlets were established on porous soil. Thus, 700 and 300 plants of cv. Takinoyosooi and Sarah Bernhardt could be theoretically obtained from a single bud in one year.Abbreviations BAP
6-benzylaminopurine
- GA
gibberellic acid
- NAA
a-naphthaleneacetic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) basal medium |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |