Changes in the binding of concanavalin A and wheat germ agglutinin to human lymphocytes during in vitro transformation |
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Authors: | U Krug M D Hollenberg P Cuatrecasas |
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Institution: | 1. Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA;2. Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA |
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Abstract: | Concanavalin A binds to human circulating lymphocytes in a complex manner suggesting the presence of multiple binding sites. Saturation of one or more of these binding sites is observed at concentrations of concanavalin A which induce blast transformation in lymphocytes. In contrast, only one saturable binding site is observed for wheat germ agglutinin. During transformation, the amount of concanavalin A which can be bound by lymphocytes increases, whereas the amount of wheat germ agglutinin which can be bound remains unchanged. Since the size increases during transformation, there must be a fall in the density of surface receptors for wheat germ agglutinin whereas the density of concanavalin A receptors remains unchanged. |
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