Disaccharide analysis of glycosaminoglycans synthesized by cardiac myxoma cells in tumor tissues and in cell culture |
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Authors: | Negishi Mitsuko Sakamoto Hironosuke Sakamaki Tetsuo Ishikawa Osamu Kanda Tsugiyasu Tamura Jun-ichi Kurabayashi Masahiko Nagai Ryozo |
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Institution: | Second Department of Internal Medicine, 3-39-22, Showa-machi, Maebashi, Gunma 371-8511, Japan. |
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Abstract: | We analyzed the main disaccharide units of glycosaminoglycans synthesized by cardiac myxoma cells in vivo and in cell culture using high-performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling. Cardiac myxoma tissues contained large amounts of chondroitin-6-sulfate (46%) and hyaluronic acid (32%), along with some chondroitin-4-sulfate (13%), chondroitin (6%), and much less dermatan sulfate (3%). Cultured cardiac myxoma cells synthesized mainly chondroitin-6-sulfate. The abundant glycosaminoglycans in myxoma tissues may make up the characteristic friable gelatinous matrix which is favorable for embolism and tumor cell growth. |
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