Molecular evolution inferred from immunological cross-reactivity of immunoglobulin G among Chiroptera and closely related species. |
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| Authors: | Tsutomu Omatsu Yoshiyuki Ishii Shigeru Kyuwa Elizabeth G Milanda Keiji Terao Yasuhiro Yoshikawa |
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| Institution: | Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. |
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| Abstract: | We examined the relationships between Megachiroptera and Microchiroptera, and between Chiroptera and other closely related species by the cross-reactivity of immunoglobulin epitopes. Rabbit polyclonal antibody to bat IgG was used for determining the cross-reactivity by a competitive ELISA method. Megachiroptera and Microchiroptera showed high cross-reactivity, over 95.3%, with each other. However, primates and insectivores showed very low cross-reactivity, 8.6 to 20.2% and 5.3 to 12.7%, respectively. These results suggest that suborders of Chiroptera are monophyletic and Chiroptera have a relatively closer relationship to primates than to insectivores. |
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