Comparative study of wild and cultivated <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> (Bangiales,Rhodophyta) based on molecular and morphological data |
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Authors: | Kyosuke Niwa Aki Kato Atsushi Kobiyama Hiroshi Kawai Yusho Aruga |
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Institution: | (1) Fisheries Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Akashi Hyogo, 674-0093, Japan;(2) Kobe University Research Center for Inland Seas, Rokkodai, Kobe Hyogo, 657-8501, Japan;(3) Laboratory of Aquatic Microbiology, School of Fisheries Sciences, Kitasato University, Sanriku, Ofunato Iwate, 022-0101, Japan;(4) Nishikamata 2-4-21, Ota-ku, Tokyo 144-0051, Japan |
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Abstract: | We compared the wild Porphyra strain OGATSU from northeastern Japan with cultivated Porphyra yezoensis f. narawaensis using the RuBisCO spacer, rbcL, and ITS-1 DNA sequences as well as early gametophyte development. Based on the molecular analyses and detailed morphological
observations, OGATSU was identified as P. yezoensis, but also revealed important differences from the cultivated form. Under the same culture conditions, gametophytic blades
of OGATSU produced more archeospores than P. yezoensis f. narawaensis strain HG-4. The length of blades and their length-to-width ratios were significantly lower in OGATSU than in HG-4, and the
color of OGATSU blades was darker than that of HG-4. The first lateral cell division in conchospore germlings occurred significantly
earlier in the OGATSU strain than in the HG-4 strain, resulting in the rounder shape of the OGATSU blade compared to that
of P. yezoensis f. narawaensis. These results suggested that wild strains such as OGATSU can provide useful characters that could enhance cultivated varieties
in a careful breeding program. |
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Keywords: | Internal transcribed spacer PCR-RFLP Molecular phylogeny Porphyra yezoensis rbcL RuBisCO spacer |
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