应用ISSR标记研究仲彬草属植物的遗传变异

利用ISSR标记对仲彬草属14个种和1个变种共32份材料进行了研究。结果表明:仲彬草属材料间ISSR标记多态性较高,变异较大。12个引物共扩增出593条带,其中535条(90.2%)具有多态性,每个引物可扩增出11~80条多态性带,平均44.6条。ISSR标记遗传相似系数在种间变化范围为0.430~0.866,平均值为0.620。从聚类分析得知,ISSR标记能将32份仲彬草属材料完全分开,32份材料聚为4类。同种不同居群的材料分别聚在一起,亲缘关系较近,同时,在分子水平上种内不同居群间也存在分化;种间存在明显的遗传差异;形态相似、地理分布一致的物种倾向于聚类在一起,有一定的亲缘关系。因此,ISSR分子标记能有效地评价仲彬草属物种的亲缘关系。

Study on genetic variation of genus Kengyilia by ISSR markers

The results of ISSR markers showed that higher genetic variation was present among the materials analyzed. A total of 593 bands were amplified from 12 primers,of which 535 bands(90.2%)were polymorphic. 11～80 polymorphic bands could be amplified from each primer,with an average of 44.6 bands. The average GS value of species was 0.620,ranging from 0.430 to 0.866. Cluster analysis showed that all materials could be distinguished by ISSR markers and classified into 4 groups. The different accessions in a species were clustered together respectively,and had genetic variation in molecular levels. Distinct genetic differences exist among the species;The species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together,and had close relationships. It is a useful method for analysis of the genetic variation in Kengyilia.