Mapping of a BYDV resistance gene fromThinopyrum intermedium in wheat background by molecular markers |
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| Authors: | Zengyan Zhang Zhiyong Xin Youzhi Ma Xiao Chen Qiongfang Xu Zhishan Lin |
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| Institution: | (1) Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing, 100081, China;(2) CSIRO Plant Industry, GPO Box 1600, Canberra, 2601, ACT, Australia; |
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| Abstract: | The wheat line H960642 is a homozygous wheat-Thinopyrum intermedium translocation line with resistance to BYDV by genomicin situ hybridization (GISH) and RFLP analysis. The genomic DNA ofTh. intermedium was used as a probe, and common wheat genomic DNA as a blocking in GISH experiment. The results showed that the chromosome
segments ofTh. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation line H960642
is a T7DS-7DL-7XL translocation by using 8 probes mapped on the homoeologous group 7 in wheat. The translocation breakpoint
is located between Xpsr680 and Xpsr965 about 90–99 cM from the centromere. The RFLP markers psr680 and psr687 were closely
linked with the BYDV resistance gene. The gene is located on the distal end of 7XL around Xpsr680 and Xpsr687. |
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