Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment |
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| Authors: | F. J. L. Aragão L. M. G. Barros A. C. M. Brasileiro S. G. Ribeiro F. D. Smith J. C. Sanford J. C. Faria E. L. Rech |
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| Affiliation: | (1) Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia, EMBRAPA, P.O. Box 02372, 70849-970 Brasília, DF, Brazil;(2) Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456 Geneva, NY, USA;(3) Centro Nacional de Pesquisa de Arroz e Feijão, EMBRAPA, P.O. Box 179, 74001 Goiânia, GO, Brazil |
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| Abstract: | Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome. |
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| Keywords: | Transgenic bean Phaseolus vulgaris Co-transformation Biolistic Inheritance |
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