Identification of several proteins involved in the messenger RNA binding site of the 30 S ribosome by inactivation with 2-methoxy-5-nitrotropone |
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Authors: | Chu Chang Gary R. Craven |
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Affiliation: | Laboratory of Molecular Biology and Department of Genetics University of Wisconsin Madison, Wis. 53706, U.S.A. |
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Abstract: | Chemical modification of unwashed 30 S ribosomal subunits with 2-methoxy-5-nitrotropone causes a rapid loss of their capacity to bind bacteriophage Qβ RNA. Reconstitution experiments show that ribosomal protein is the functionally inactivated species. When purified unmodified ribosomal proteins were included in a mixture of 16 S ribosomal RNA and total protein derived from 2-methoxy-5-nitrotropone-treated subunits, four proteins (S1, S12, S13 and S21) were found to promote the reconstitution of particles capable of binding natural messenger RNA. |
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Keywords: | MNT 2-methyl-5-nitrotropone |
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