Activated oxygen ashing of biological specimens for the microdetermination of Na, K, Mg, and Ca by atomic absorption spectrophotometry |
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| Authors: | H Sanui |
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| Affiliation: | 1. Mechanical Engineering Department, Khalifa University, Abu Dhabi, United Arab Emirates;2. System on Chip Lab, Khalifa University, Abu Dhabi, United Arab Emirates;3. Laboratoire de Physique Quantique de la Matière et Modélisation Mathématique, University of Mascara, Algeria;4. Department of Mechanical Engineering, College of Engineering in Wadi Alddawasir, Prince Sattam Bin Abdulaziz University, Saudi Arabia;1. Graduate School of Environmental Studies, Nagoya University, Japan;2. Department of Civil and Environmental Engineering, Imperial College, London SW7 2AZ, UK;3. Marine Explorers Society – 20000 Leagues, Put Bokanjca 26A; 23000 Zadar, Croatia;1. CONSTRUCT-LFC, Faculty of Engineering (FEUP), University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal;2. CI&DETS - Polytechnic Institute of Viseu, School of Technology and Management, Department of Civil Engineering, Campus Politécnico de Repeses, 3504-510 Viseu, Portugal |
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| Abstract: | A procedure employing electrically activated oxygen for the oxidation of organic substances at relatively low temperatures (100–125°C) and requiring no large additions of chemical reagents is applied to the destruction of biological materials preliminary to elemental analyses by atomic absorption spectrophotometry. Results of recovery studies for micromolar levels of Na, K, Mg, and Ca in rat liver cell microsomes are highly satisfactory when specially designed quartz ashing chambers are used with the Tracerlab LTA-500A low temperature asher. Activated oxygen ashing appears to offer a unique and superior method for the treatment of milligram amounts of biological materials preliminary to elemental analyses of samples containing micromolar levels of biologically important elements. |
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