A functional proteomics approach for the detection of nuclear proteins based on derepressed importin alpha |
| |
Authors: | Blazek Erik Meisterernst Michael |
| |
Affiliation: | Department of Gene Expression, National Research Center for Environment and Health-GSF, Institute of Molecular Immunology, Munich, Germany. |
| |
Abstract: | The identification of functional proteomes is a major challenge in proteomic research. Here we describe a method for the detection and isolation of nuclear (localization sequence containing) proteins using a derepressed import receptor (DIRE) as a synthetic antibody. We demonstrate that the DIRE method specifically detects nuclear localization sequence containing proteins. Application to activation of primary T-lymphocytes exemplifies the potential use of DIRE for comparative proteomics and for diagnostics. |
| |
Keywords: | Far‐Western Importin NLS Nuclear proteins Nuclear proteome |
本文献已被 PubMed 等数据库收录! |