Ischemic but not mechanical preconditioning attenuates ischemia/reperfusion induced myocardial apoptosis in anaesthetized rabbits: The role of Bcl-2 family proteins and ERK1/2 |
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Authors: | Antigone Lazou E K Iliodromitis D Cieslak K Voskarides S Mousikos E Bofilis D T Kremastinos |
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Institution: | (1) Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece;(2) 2nd University Department of Cardiology, Medical School, Attikon General Hospital, University of Athens, Athens, 124 62 |
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Abstract: | Objective: Recent studies suggest that ischemic preconditioning (IPC) inhibits myocardial apoptosis after ischemia and reperfusion.
This study aimed first, to examine whether short mechanical stretch with acute pressure overload (MPC), which has been shown
to reduce infarct size after ischemia/reperfusion, mimics IPC in attenuating myocardial apoptosis and second, to evaluate
whether induced cardioprotection involves modulation of the expression of the Bcl-2 family proteins and phosphorylation of
prosurvival kinases. Methods and Results: A model of anaesthetized rabbit was used and the preconditioning protocol included one cycle of short ischemia/reperfusion,
or short mechanical stretch with acute pressure overload. Preconditioning stimuli were equally effective in reducing the infarct
size, determined after 4 h reperfusion. However, IPC but not MPC attenuated myocardial apoptosis. IPC restored the decreased
expression of Bcl-2 and Bcl-xL observed in hearts subjected to ischemia and reperfusion only. Bax levels were not different
among the groups. ERK1/2 were activated during reperfusion in both IPC and MPC groups. Conclusions: The data provide further evidence that apoptosis and necrosis contribute independently to infarct size after ischemia and
reperfusion. Inhibition of the myocardial apoptotic processes by IPC may involve modulation of the expression of anti-apoptotic
proteins, Bcl-2 and Bcl-xL. ERK1/2 may be involved in the inhibition of both apoptosis and necrosis. |
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Keywords: | Apoptosis Bcl-2 family Infarct size ERK1/2 Ischemia/reperfusion Preconditioning Rabbit heart |
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