Abscisic acid and proline improve desiccation tolerance and increase fatty acid content of celery somatic embryos |
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Authors: | Yong-Hwan Kim Jules Janick |
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Affiliation: | (1) Department of Horticulture, Purdue University, 47907 West Lafayette, IN, USA;(2) Agricultural Sciences Institute, RDA, 440-707 Suwon, Korea |
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Abstract: | Desiccation tolerance of celery (Apium graveolens L.) somatic embryos was increased by supplementation of embryo-production medium with 1 M abscisic acid (ABA) or 1 mM proline, with highest survival obtained with a combination of 1 M ABA and 1 mM proline. Addition of ABA and proline increased fatty acid accumulation by somatic embryos; the effect on fatty acid composition was inconsistent. Somatic embryos capable of germination differed from mature zygotic embryos by greater size, lower fatty acid level, and substantially lower proportion of oleic acid (18:1) as compared to linoleic acid (18:2). |
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Keywords: | abscisic acid Apium graveolens celery desiccation fatty acids proline somatic embryos zygotic embryos |
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