High-cell-density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source |
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Authors: | P A E P Meesters G N M Huijberts G Eggink |
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Institution: | (1) Agrotechnological Research Institute (ATO-DLO), P.O. Box 17, 6700 AA Wageningen, The Netherlands. Fax: 31 8370 12260, NL |
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Abstract: | Cryptococcus curvatus is a yeast with industrial potential because it can grow and accumulate lipid on a very broad range of substrates. In this
study we describe growth and lipid accumulation on glycerol in a fed-batch fermentation mode. We performed a fermentation
consisting of two phases. The first phase is the biomass production phase in which there is no nutrient limitation except
for very short periods of glycerol exhaustion. The substrate feed was controlled by the dissolved oxygen tension. In the second
phase nitrogen limitation was introduced, which causes lipid accumulation. This way very high cell densities of 118 g/l in
a 50-h fermentation could be reached. With a lipid production rate of 0.59 g lipid l-1h-1, a cellular lipid content of 25% was obtained. The growth and lipid accumulation phase are characterized by different cellular
fatty acid compositions. In the growth phase, a relatively high amount of C18:2 (linoleic acid) is present, which is a major
component of membrane lipids. C18:0 (stearic acid) and C18:1 (oleic acid) are major constituents of the accumulated triglycerides
and therefore the relative amount of C18:2 decreases during the lipid accumulation phase.
Received: 19 September 1995/Received revision: 28 December 1995/Accepted: 8 January 1996 |
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