16S rDNA克隆文库方法分析太岁样品中细菌的多样性

通过构建16S rDNA克隆文库的方法,分析太岁样品中细菌的群落结构及多样性。太岁样品中的细菌归属于4个门9个目,优势类群依次是芽胞杆菌目(Bacillales,33.01%)、柄杆菌目(Caulobacterales,32.04%)和伯克霍尔德氏菌目(Burkholderiales,12.62%);优势属为短波单胞菌属(Brevundimonas,30.10%)、葡萄球菌属(Staphylococcus,29.13%)和食酸菌属(Acidovorax,7.77%)。并且其中的5个目中含有未培养的细菌,红杆菌目(Rhodobacterales)、伯克霍尔德氏菌目和红环菌目(Rhodocyclales)的11个克隆子的细菌16S rDNA序列同源性低于97%。研究表明太岁样品中细菌多样性较丰富,且蕴藏着许多未知的微生物资源。

Analysis of Bacterial Diversity by 16S rDNA Clone Library from Taisui Sample

The bacterial community structure and diversity of Taisui sample were analyzed through the construction of 16S rDNA clone libraries. Bacteria in Taisui sample belonged to 4 phyla and 9 orders. The dominant orders of bacteria were Bacillales (33.01%), Caulobacterales (32.04%) and Burkholderiales (12.62%). The most dominant genus was Brevundimonas(30.10%), the secondary dominant genus was Staphylococcus(29.13%), followed by Acidovorax (7.77%). There were uncultured bacteria of 5 orders among them, and 11 clones from Rhodobacterales, Burkholderiales and Rhodocyclales of bacterial 16S rDNA sequence homology were less than 97%. The results indicated that high bacterial diversity was found in Taisui, also there were many unknown microbial resources.