High-level expression of a xylanase gene from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris |
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| Authors: | Guangsen Fan Priti Katrolia Huiyong Jia Shaoqing Yang Qiaojuan Yan Zhengqiang Jiang |
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| Institution: | 1. Department of Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Haidian District, A21, P.O. Box 294, Beijing, 100083, China
2. Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, 100083, China
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| Abstract: | A xylanase gene from Paecilomyces thermophila was functionally expressed in Pichia pastoris. The recombinant xylanase (xynA) was predominantly extracellular; in a 5?l fermentor culture, the total extracellular protein was 8.1?g?l?1 with an activity of 52,940?U?ml?1. The enzyme was purified to homogeneity with a recovery of 48?%. The recombinant xynA was optimally active at 75?°C, as measured over 10?min, and at pH 7. The enzyme was stable up to 80?°C for 30?min. It hydrolyzed birchwood xylan, beechwood xylan and xylooligosaccharides to produce xylobiose and xylotriose as the main products. |
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