An improved method for determining the activity of benzoyl-arginine p-nitroanilide-hydrolyzing enzyme in crude tissue extracts |
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Authors: | H Nakagawa K Shuto S Tsurufuji |
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Affiliation: | Department of Biochemistry Faculty of Pharmaceutical Sciences Tohoku University Aoba, Aramaki Sendai 980, Japan |
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Abstract: | The method described in this communication is sensitive and allows the direct determination in crude tissue extracts of the enzyme that hydrolyzes α-N-benzoyl-dl-arginine p-nitroanilde (Bz-Arg-NPhNO2). It may be useful in determining cathepsin B activity in crude enzyme preparations when the reaction mixture is incubated with an inhibitor of trypsinlike enzymes which are also capable of hydrolyzing Bz-Arg-NPhNO2. |
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