Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase |
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Authors: | Zhao Genhai Liu Junzhong Dong Kai Zhang Fei Zhang Hongjuan Liu Qian Jiao Qingcai |
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Affiliation: | State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, People's Republic of China. |
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Abstract: | An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HHW) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40°C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1). |
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